Spark®

The Tecan Spark® Multimode Microplate Reader is a versatile, fully configurable detection platform designed to support a wide range of life science research and drug discovery applications. Combining multiple detection technologies in one instrument, it enables absorbance, fluorescence, time-resolved fluorescence, FRET, TR-FRET, fluorescence polarisation and luminescence measurements. Optional modules extend its capabilities to automated cell imaging, live-cell analysis, reagent injection and advanced environmental control. Its innovative optical technologies, including High Speed Monochromators and Fusion Optics, provide flexibility for sensitive measurements and rapid spectral analysis. Controlled through intuitive SparkControl™ software, Spark offers adaptable workflows and upgradeable configurations to meet changing laboratory requirements.

Description

Features

Fully configurable multimode microplate reader platform.
Absorbance measurements, including UV/VIS spectral scanning from 200–1,000 nm.
Fluorescence intensity detection from top and bottom.
Time-resolved fluorescence (TRF), FRET, TR-FRET and fluorescence polarisation.
High-sensitivity luminescence detection, including glow, flash and multicolour applications.
Support for AlphaScreen®, AlphaLISA® and AlphaPlex® assays.
Fusion Optics® combining monochromator flexibility with filter sensitivity.
Optional automated cell imaging for cell counting, viability and confluence assessment.
Optional live-cell environmental controls, including temperature, CO₂ and O₂ regulation.
Te-Cool™ active temperature control from 18–42°C on equipped configurations.
Optional reagent injectors for automated dispensing and kinetic measurements.
Compatibility with 6- to 1,536-well microplates, depending on measurement mode and configuration.
Optional Spark-Stack™ plate stacker for automated processing of up to 50 plates per run.
SparkControl™ software with one-click applications and workflow-based operation.

Benefits

Consolidates multiple detection technologies in a single instrument.
Supports a broad range of biochemical, cellular and molecular research applications.
Flexible configuration allows the reader to be tailored to laboratory requirements.
Upgradeable design provides scope to add capabilities as research needs evolve.
Sensitive optical technologies support reliable detection of low-intensity signals.
Rapid spectral scanning helps reduce measurement time and sample evaporation.
Automated imaging supports cell counting and cell-based assay analysis.
Environmental control enables long-term live-cell kinetic studies.
Reagent injection supports the measurement of rapid biological reactions.
Automated plate handling helps increase throughput and reduce manual intervention.
Intuitive software simplifies method setup and routine instrument operation.