HydroFlex Microplate Washer

HydroFlex Microplate Washer

Multimode Reader

The HydroFlex microplate washer is a truly flexible platform that offers excellent automated microplate strip washing and vacuum filtration performance for 96-well microplate formats.

This modular and upgradeable platform is ideal for a wide range of cell-, enzyme- and DNA-based applications in academia, biotech, pharma and clinical diagnostics, reflecting over 30 years of Tecan expertise in advanced liquid handling.

Integrated application flexibility

The multifunctional HydroFlex microplate washer offers far greater flexibility than a standard plate microplate washer, and features user-interchangeable plate carriers for easy switching between washing and filtration applications to allow:

  • ELISA washing for improved consistency and reliability.
  • Gentle washing of cell-based assays with adherent or weakly adherent cells.
  • Microplate array washing for protein and antibody arrays.
  • Vacuum filtration to waste for PCR clean up after DNA amplification, etc.
  • Magnetic bead washing using MBS carrier.

Ready for robotic integration

Every HydroFlex plate washer is ready for integration onto the following automated platforms.

  • Freedom EVOlyzer® for ELISA automation
  • Freedom EVO®

  • Fluent®


  • Compact and modular design, suitable for a range of applications and laboratories
  • Ready for automated washing of ELISAs, cells and protein arrays
  • Automated system for vacuum filtration to waste
  • Magnetic plate carrier for magnetic bead washing
  • Advanced online process controls for safety and reliability
  • Bubble sensor unit that ensures reliable buffer dispensing
  • Multipoint aspiration for flat bottom plates to achieve minimal residual volumes
  • Individual software control of speed settings and wash head positions
  • Suitable for integration onto Freedom EVO and Freedom EVOlyzer workstations
  • Excellent reliability and low service costs


ELISA and cell washing*

Using adjustable wash settings and head positions for optimized results.

  • For gentle washing of adherent and weakly adherent cells
  • For efficient ELISA washing and minimum residual volumes


Magnetic bead washing

Using a patented design with two neodymium magnets per well.

  • For efficient bead settling and good bead retention
  • Linear plate shaking and strong dispensing for mixing of beads


Vacuum filtration to waste

Using tunable vacuum for PCR clean-up and bead washing.

  • Automation-friendly solution for self-sealing of filtration plates
  • Optimized vacuum settings for each filtration step within a protocol



The HydroFlex can be fully programmed and operated as a standalone instrument using the built-in display and keypad, and features a selection of predefined cleaning protocols for easy maintenance.

Every HydroFlex system is also supplied with Tecan’s intuitive HydroControl software, providing example wash programs and a predefined plate library, as well as a simulation mode for offline operation. Wash programs created in HydroControl can be quickly transferred to the on-board memory of the HydroFlex plate washer via a USB interface, using the download function.


  • The HydroFlex plate washer has been designed to meet IVD-D 98/79/EC when configured for ELISA washing*
  • In combination with the HydroControl software, HydroFlex provides 21 CFR Part 11 functionality, such as electronic records, electronic signatures, user administration and audit trail functions



General Specifications
Display unit Liquid crystal display with two rows of 16 digits
Keyboard Four-key membrane keyboard
Number of dispensing channels 1, 2 or 4 channels
Manifold types 8- or 16-way for parallel processing of 8- or 16-wells
Volume of solution dispensed 50-3,000 µl in 50 µl steps (washing)
Dispensing accuracy ≤1.2 % typical (300 µl dispense volume, dispense rate: 3, 8-way manifold)
Dispensing uniformity CV ≤2.5 % over the plate typical (300 µl dispense volume, dispense rate: 3, 8-way manifold)
Residual volume Crosswise aspiration: ≤1.8 μl per well typical (96-well, flat-bottom Greiner® Bio-One plate) (asp. rate: 3; asp. time: 4 sec; head speed: 10 mm/sec; 8-way manifold)
Vacuum range High-level vacuum filtration: -150 to -850 mBar absolute pressure
Low-level vacuum filtration: -50 to -150 mBar absolute pressure
Interface USB
Shaking Linear shaking; high, medium and low settings
Supply Auto sensing for the settings 100-120 V or 220-240 V, 50/60 Hz
Consumption 65 VA
Outside dimensions for plate washing Width: 27.5 cm; depth: 36.6 cm; height: 18.5 cm
Weight 6.6kg
Colour White (standard),Metallic silver, Metallic red, Metallic blue, Metallic orange, Lime green