Spark Cyto

Spark Cyto

Spark Cyto is a mutli-mode plate reader combining fluorescence imaging and cytometry capabilities. By combining live cell imaging and detection technologies, researchers now have the ability to combine qualitative and quantitative information into multiparameter data sets.

Spark Cyto works in real time using parallel data acquisition and analysis in order to deliver insights faster than ever before.

With four specialised configurations with differing detection modalities, there is always a system to suit your needs.



  • Nuclei counting
  • Cell viability
  • Apoptosis
  • Confluence assessment
  • Cell migration & wound healing
  • ELISAs
  • DNA/RNA quantification
  • Nucleic acid labelling efficiency
  • Protein quantification
  • Reporter Gene Assays
  • BRET & NanoBRET®

Detection Modes:

  • Fluorescence imaging (blue, green, red, far red)
  • Brightfield imaging
  • Digital Phase Contrast-imaging
  • Absorbance – including UV/VIS
  • Fluorescence top & bottom
  • Full spectral scanning capabilities for all measurement modes
  • FRET
  • Fluorescence polarisation
  • Luminescence- glow, flash multicolour, scanning
  • AlphaScreen®, AlphaLISA® & AlphaPlex®

Features & Benefits


Features Benefits
Built-in objective changer with 2X, 4X & 10X objectives Seamless selecting of multiple magnitudes during experiments
5 LEDs (brightfield, digital phase contrast & fluorescence excitation) Multiple channels for detection and imaging
Multiband filter set Detection range from 381-800nm
12-bit CMOS camera Whole well imaging in 96 or 384 well-plates
Composite images with no pixel shift Remarkable image quality & capture speed
LED-based Autofocus Accurate images without compromising on scanning time
Live Viewer™ Real time monitoring

Add-Ons & Accessories

  • Enables walkaway automation for live cell experiments up to 40 plates
  • Lid-lifting technology in Spark Cyto protects plates outside the incubator, saving on costs for safety cabinets
  • Expandable with additional instrumentation


Spark-Stack™ microplate stacker

A compact, automated plate stacker for integration with Spark readers, providing reliable walkaway processing of upto 50 plates per run

Injectors with Heating & Stirring

Integrated injectors permit automated reagent dispensing, ideal for high sensitivity applications such as flash luminescence. An attachable heater/stirrer unit reduces the risk of precipitation.

Humidity Casette


  • Reduction in evaporation during your assay
  • Use your existing plates – no need for expensive special plates
  • Better data quality – consistant results
  • Reduced edge effects – ideal for long-term kinetics
  • Stable conditions – better cell viability

Tecan microplates

A collection of tissue-culture (TC) treated, sterile cell culture plates with individual lids for cell imaging and bottom-fluorescence measurements of cell-based applications. Performance assured with minimum background signal.